What is cryopreservation?
Cryopreservation, it is the procedure of freezing biological material at extreme temperature, most commonly -196 °C/-321 °F in liquid nitrogen. At this very low temperature, all biological activity stops the biochemical reactions that lead to the death of cell and degradation of DNA. The infertility specialist in Hyderabad, who are working in the Motherhood fertility centre, the leading fertility centre in Hyderabad find out some theories which make this cryopreservation method possible to store living cells along with other biological material unchanged for centuries. Because of the investigations now Motherhood fertility centre is becoming the best Infertility Hospital in Hyderabad providing the best Infertility Treatment in Hyderabad.
The main challenge for the infertility specialist in Hyderabad of cryopreservation is to help cells to survive both codings to the extreme level of temperatures and thawing back to some physiological conditions. Particularly, intercellular ice formation is a very critical issue which has to be controlled to keep the cell membrane intact and the cells alive. The important elements to prevent this are the freezing rate and the consumption of the freezing medium utilized. Generally, this freezing medium consists of a diluter, which is a protein source and cryoprotectant compound. The choice of the most suitable cryoprotectant will give impact to the preservation result and will be much different between the cells and different species.
For the infertility specialist in Hyderabad, cryopreservation is very much important in breeding programs to reserve desired genes but provides an opportunity to save endangered species. Cryopreservation is performed by the cryogenetics in special laboratories of best fertility centers in Hyderabad. Cryogenetics is the process which includes all the steps from quality evaluation, packing, dilution and freezing in liquid nitrogen.
After the sperm is cryopreserved in liquid nitrogen, the couple can choose how you would like the cells to be stored with us or if you want the cells can be returned for storage at your own site. Only you require to notify cryogenetics when the material is required for the fertilization process and then we will organize the return of shipment. The infertility specialists in Hyderabad working in the best fertility centers in Hyderabad get training for the milt thawing and fertilization process.
Having the exact cryopreservation protocol is having the best possible container in which to store the frozen sperm. Each container requires to be tailored by the infertility specialist in Hyderabad to its application; an optimal volume, biosecure, non-toxic, space-saving, practical and aid the fertilization procedure after thawing. Based upon these design principles, Cryogenetics has evolved two specialized and patented receptacles. Those are SquarePack® and a “Micro-straw”.
The SquarePack® volume is about 12.5 ml and is designed for freezing the cells of milt in large volumes. This is a critical factor in providing efficient reproduction and would be utilized for Atlantic salmon for example. One salmon male can provide more than 1 liter of diluted milt which means that about 0.5 ml straws are far too small for such larger scale production requirements.
The “Micro-straw” was developed at the opposite end of the scale, like Zebrafish who provide sperm in a volume of nearly 1-3 µl. The “Micro-straw” holds volumes about 50 µl of diluted sperm which is a more suitable container for these smaller species.
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